Univ. of NE-Lincoln: Effects of Crystalline Lactose on Expression

A preliminary experiment was conducted to evaluate the effects of crystalline lactose on the relative abundance of sodium-dependent glucose transporter (SGLT)-1 mRNA in vitro in model porcine jejunal epithelial cells (IPEC-J2). Cells were treated with low (28 mM) or high (56 mM) concentrations of lactose alone or in combination with lipopolysaccharide (LPS; 10 ng/mL). Total RNA and culture media samples from both apical and basolateral compartments were harvested at 3, 6, 12, and 24 hours following the addition of respective treatments.